Journal: BMC Genomics

Article Title: Systematic analysis of T7 RNA polymerase based in vitro linear RNA amplification for use in microarray experiments

doi: 10.1186/1471-2164-5-29

Figure Lengend Snippet: Comparison of generalised log ratios in hybridisations on 7,347-gene microarrays Each boxplot (x-axis) characterises the distribution of the generalised log ratios (y-axis) of A) total RNA vs. total RNA and aRNA vs. aRNA from the same origin (duplicate hybridisations of Ta, Tb and Aa, Ab, respectively) and from different tissue sources (triplicate hybridisations of Tc, Td and Ac, Ad, respectively) and B) 15 microarray hybridisations from the same tissue sources using total RNA vs. aRNA (A) and 14 hybridisations using aRNA vs. aRNA (B and C).

Article Snippet: The 30 μl probe was heat denatured at 65°C for 2 min and hybridised to cDNA glass microarrays in a hybridisation chamber (Corning Inc., Acton, USA) over night at 37°C.

Techniques: Microarray

Journal: Developmental biology

Article Title: PRDM paralogs are required for Meckel’s cartilage formation during mandibular bone development

doi: 10.1016/j.ydbio.2025.12.002

Figure Lengend Snippet: (A,B-A””,B””) Hybridization chain reaction (HCR) was performed on coronal sections of E10.5 mandibular processes to assess gene expression patterns for Dlx5 (green) (A-B), Dlx6 (cyan) (A’-B’), Hand2 (magenta) (A”-B”), Sox10 (yellow) (A”’-B”’) in controls and double mutants. (A””-B””) Merged image of A-A”’ and B-B”’ with the counterstain DAPI in blue. (C) RT-qPCR for the indicated mRNA transcripts isolated from the mandibular processes of control and Prdm3;Prdm16 double mutants at E10.5 (n = 4 for both control and mutants). (D) RT-qPCR for Sox10 at E10.5 (n = 4 for both control and mutants) and E11.5 (n = 3 for control, n = 4 for mutants). (E-F, E’-F’) HCR was performed on coronal sections of E11.5 mandibular processes to assess gene expression patterns for Sox10 . (E’-F’) Merged image of E and F with the counterstain DAPI in blue. Double white arrowhead denotes the initial presumptive cranial ganglia and peripheral nerve tracks entering the mandibular prominence in control animals. White arrow indicates areas of Sox10 expression that are expanded in the mesenchyme in the Prdm3;Prdm16 double mutants. Abbreviations: MdP, mandibular process. *P≤0.05; (unpaired, two-tailed Student’s t test).

Article Snippet: Sections were pre-hybridized in HCR HiFi Probe Hybridization Buffer (Molecular Instruments) for 10 minutes at 37°C in a humidified chamber.

Techniques: Expressing, Hybridization, Gene Expression, Quantitative RT-PCR, Isolation, Control, Two Tailed Test